Accreditation means that we have been assessed against internationally recognised standards and operate to the highest levels of quality and service.

The section’s primary goal is to assist researchers requiring instrumentation and expertise in Fermentation Technology for protein (recombinant or native), biomass and chemical production. Our services range from PCR, construction of expression systems to fermentation optimisation. Researchers can access to our facilities through contract research or rental, for both research and pilot-scale studies.

KEY INSTRUMENTATION:

SERVICES:

● Molecular cloning (PCR, construction of bacterial & yeast expression systems)
● Protein / Biomass / Chemical production (up to 20 litres per run)
● Facility Rental
● PEB Lab Attachment
● PEB Individual / Group Training

The section offers a range of techniques for identification, characterization and quantification of proteins, peptides and metabolites. The facility is equipped with a high-resolution mass spectrometer and advanced data analysis software.

KEY INSTRUMENTATION:

–  nanoLC coupled with Orbitrap Fusion (LCMS/MS)
–  UHPLC coupled with Single Quadrupole Detector (SQD)

DATA ANALYSIS SOFTWARE

•       Proteome Discoverer for Identification & Quantification Analysis
•       MarkerLynx and MetaboLynx for Metabolite & Biomarker Analysis
•       DeNovoGUI for De Novo peptide sequencing
•       QuanLynx for Quantitative Analysis
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SERVICES:

PROTEOMICS SERVICES

Protein Digestion

•   Protein has to be cut enzymatically into smaller peptides before the LCMS/MS analysis.
•  Mixture of Trypsin-LysC enzyme is used to digest the protein.
• The digestion process can be performed either in-solution or in-gel.

Protein Identification

• Digested peptides are analysed using the on-line reversed phase nanoLC coupled with Orbitrap Fusion.
•  The data analysis software is used to search the acquired MS/MS spectra to identify the proteins. The software will confirm the identified protein and the post-translational modifications if present.

Relative Protein Quantification

• Proteins expression in different samples treatment can be compared relatively using an isotope labeling or label-free approach.
• The data analysis software is used to generate a peak area (intensity) of identified protein for a statistical analysis.

De Novo Peptide Sequencing

• Peptide of interest in the range of 10-15 amino acids can be sequenced using mass spectrometer.
• Sequencing service includes pre-acquisition prior to mass spectrometer and possible sequence will be confirmed by fragmentation data (MS/MS).

METABOLOMICS SERVICES

The Metabolomic service is equipped with a single quadrupole detector mass spectrometry coupled with UHPLC to separate and detect a targeted molecular mass. This equipment is an easy way to generate an extra dimension of information from the separation and capable of generating fast result molecular mass though direct sample injection. The services we offer are:

• Analysis of known compound or targeted compounds based on molecular mass
• Nominal mass determination
• Peak purity and impurity profiling
• Syntheses confirmation
• Mass-based peak of interest molecule identification 
• Quantitative Analysis
• Direct Infuse Mass Spectrometry (DIMS)

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The STS provides researchers a complete genomics solution using Next-Generation Sequencing (NGS) and Sanger Technology platforms. The facility can cater projects for whole genome sequencing (WGS), transcriptome sequencing, targeted sequencing, and metagenomic sequencing.

KEY INSTRUMENTATION:

SERVICES:

● Whole Genome Sequencing
● Transcriptome Sequencing
● Targeted Sequencing (amplicon-based)
● Whole Metagenomics Sequencing Package (inclusive of DNA extraction, library preparation, sequencing and data analysis)

The SBB is equipped with technologies for high-resolution structural studies of macromolecules, biomolecular interaction studies, and biophysical characterizations. We offer options for these services to be done as contract research or through facility access to researchers to suit their needs.

KEY INSTRUMENTATION:

SERVICES:

● Protein purification and characterization
● Protein crystal screening and data collection
● Biophysical characterization and molecular interactions
● 1D, 2D, 3D NMR experiments
● NMR-based metabolomics analysis

The GCB provides computing support for large-scale bioinformatics – genomics, transcriptomics, proteomics and integration analyses. Our bioinformatics scientists specialize in various areas including RNA expression, comparative genomics, molecular dynamics and structural studies, with years of experience in their respective sub-fields.

KEY INSTRUMENTATION:

• SGI UV 2000
• SGI Infinite Storage 5500

SERVICES:

● Genome Assembly and Annotation
● Structural Modeling
● SNP Calling
● Transcriptome and Differential Expression Analysis
● Phylogenetics
● Metagenomics Analysis
● Miscellaneous Sequence Analyses
● Researcher Attachment (Access to GCB facilities)
● Individual & Group Training

The GBS is a biorepository facility designated to store and manage biospecimens. We optimally store samples in a controlled access room consisting of 8 units of Ultralow -80°C Freezers and 9 units of -20°C Freezers with round-the-clock monitoring. We serve clients from both research communities and industrial partners.

KEY INSTRUMENTATION:          

       
– Ultralow -80 °C Freezers
– -20 °C Freezers
– Integrated Temperature Monitoring System

SERVICES:

● Long-term biospecimens storage for cells (bacteria, yeast, fungi), genetic vectors (DNA & RNA), and proteins.
● Freezer rentals for biospecimens.

The SBC provides solutions in research and services using advanced molecular biology approaches. Our long-term purpose includes development of novel biotechnology processes for the production of biochemicals, biomaterials, and bioenergy.

SERVICES:

● Site-directed mutagenesis
● Genomic modification
● Vector constructio

The TRPD offers services targeted towards lab-to-market transition efforts, including contract research, product analysis and consultation, and prototype development. Our clients come from diverse backgrounds inclusive of research communities, universities, companies, individuals, and industrial players.

KEY INSTRUMENTATION:

•       Mammalian cell culture facility
•       Potentiostat
•       TECAN Microplate Reader
•       CFX96 Touch qPCR

SERVICES:

– Contract Research
• Development of biosensors based on customer’s target of choice
• Mammalian cell-based study
• Antioxidant property determination
• Metabolite profiling
• DNA, RNA, PCR qualitative and quantitative assays
• Customized research based on customer’s needs
– Product Analysis & Consultation
• Material Profile Data Sheet development
• Stingless bee and bee farming strategy
• Pilot-scale cosmetic product formulation and production
• Product packaging and design consultation
– Prototype Development
• Translation of in-house research output
• Electrochemical based detection biosensor development
• Lab-on-chip
• Project continuation from Contract Research or Product Analysis and Consultation