Equipment and Intruments

INFORS BioreactorsProduces protein/biomass/chemical of interest in 2 to 20 litres of microbial culture under controlled conditions.DETAIL
INFORS Multitron Shaking IncubatorsFor incubating samples requiring constant shaking or agitation. Ideal for cell culture, cell aeration and solubility experiments.DETAIL
SARTORIUS Crossflow Filtration UnitFor large-scale cell harvesting as well as to concentrate extracellular proteins.DETAIL
EPPENDORF 5810R CentrifugeFor separating components of a liquid.DETAIL
IKA T25 Ultra Turrax HomogenizerDispersing device that can be used for homogenization, emulsification and suspension of various samples.DETAIL
BRANSON Digital SonifierDisrupts cells, bacteria, spores, or tissue. Also ideal for initiating and accelerating chemical, biochemical, and physical reactions, as well as for de-gassing liquids.DETAIL
nanoLC coupled with Orbitrap Fusion (LCMS/MS)DETAIL
UHPLC coupled with Single Quadrupole Detector (SQD)DETAIL
Data analysis softwareProteome Discoverer for identification & quantification analysis, MarkerLynx and MetaboLynx for metabolite & biomarker analysis, DeNovoGUI for de novo peptide sequencing, QuanLynx for quantitative analysis.DETAIL
Illumina MiSeq® SystemNext Generation Sequencer with up to 15Gb throughput of data per run.DETAIL
Applied Biosystems 3730XL Genetic AnalyzerCapillary-based Sequencer and Fragment Analyzer.DETAIL
Agilent 2100 BioanalyzerChip-based capillary electrophoresis systemDETAIL
Roche LightCycler® 480Real-time PCR.DETAIL
Confocal Laser Scanning Microscope SystemThe system is equipped with high performance apochromatic objective lenses to enhance the spectral imaging techniques to perform a 32-channel image acquisition with resolution of 512×512 pixels in 0.6 second per scan.
Four fluorescence channels with 3 lasers can be run simultaneously.
Imaging functions ranges from brightfield, DIC and a selection of fluorescent channels as described below:
● DAPI – Excitation: 340 – 380 nm Emission: 435 – 485 nm
● FITC- Excitation: 465 – 495 nm Emission: 515 – 555 nm
● TRIT C- Excitation: 528 – 553 nm Emission: 590 – 650 nm
● Texas Red- Excitation: 532 – 587 nm Emission: 608 – 683 nm
Confocal laser excitation wavelengths of 405 nm, 488 nm, 561 nm and 640 nm are also available.
Image acquisition of both fixed specimens and live cells are supported either in two-dimensional (2D), 3D or 4D formats.
Services are offered as follows:
● Instrument rental by hour
● Customised Image acquisition by trained personnel – in plate or slide format
High Content Screening SystemThe High Content Screening System (HCS) integrates microscopy, image processing, and high resolution visualisation tools to extract data from cells and report quantitative results. HCS employs fluorescence imaging of samples in high throughput format as well as sophisticated analysis software to allow quantitative measurements of multiple cell populations in the course of an experiment.
Services are offered in biological research and drug lead screening to identify biological substances such as natural compounds, peptides, or other active molecules that may alter the phenotype of a cell in:
● Cell morphology analysis
● Protein localisation
● Protein expression
● Cell cycle study
● Object intensity
● Granularity
● Nuclear translocation
● Neurite outgrowth detection
● Membrane translocation
● Micronuclei
Inverted, Phase Contrast Fluorescence MicroscopeThe IX71 inverted research microscope is used for advanced live cell applications. The IX71 features a modular frame and optical design that provides nine access ports for multiple input and output devices, making the system ready for multi-wavelength, advanced fluorescence applications. This fluorescence microscope efficiently detects even faint fluorescence signals without damaging the cell, optimizing multi-color observation. Delivering unprecedented image quality over a super wide light spectrum. DETAIL
Inverted Phase Contrast Microscope with CameraThe equipment is used for basic live cell culture applications such as observing cell morphology.

Field Emission Scanning Electron MicroscopeField Emission Scanning Electron Microscope (FESEM) is an imaging equipment to obtain topographical information from sample surface and surface structures including surface exposed by fracturing. Researchers in biology, chemistry and physics apply this technique to observe structures that may be as small as 1 nanometer (= billion of a millimeter). DETAIL
Flow CytometerFlow cytometry is a fluidics and laser-based technology used to analyze and quantitate extracellular proteins and DNA singly or in combination present in single cells suspension. This instrument is compatible with cells originating from multiple species including, but not limited to, human, non-human primates, rodents, and canine. DETAIL
Extracellular Flux AnalyzerThe Extracellular Flux Analyzer can be used to simultaneously interrogate the two major energy producing pathways of the cell, i.e. the mitochondrial respiration and glycolysis, in a 24-well microplate, in real-time. This instrument determines in vitro oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of cells upon drug treatment. DETAIL
HolomoniterThe equipment is used as time-lapse cytometry instrumentation for long-term quantitative analysis of living cell dynamics that circumvent the drawbacks of traditional methods requiring toxic stains. The equipment is placed inside a CO2 incubator which provides suitable conditions for mammalian cell growth. DETAIL
Automated Cell CounterThe equipment is used for counting of mammalian cells in one simple step using sophisticated cell counting algorithm to produce accurate cell counts in less than 30 seconds (with and without trypan blue staining). DETAIL
Molecular ImagerThe ChemiDoc XRS+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab™ software to optimise imager performance for fast, integrated, and automated image capture and analysis of various samples. DETAIL
Multimode Plate ReaderThe equipment is used to detect biological, chemical or physical events of samples in microtiter plates. This multimode plate reader available at IPharm is for detection of (fluorescence intensity (including FRET), fluorescence polarisation, luminescence (including BRET), UV/Vis absorbance, time resolved fluorescence (including TR-FRET) and AlphaScreen®/ AlphaLISA®) in various plate formats. DETAIL
Multi-Mode Microplate ReaderSynergy™ HTX is designed for samples read out from 6- to 384-well microplates and Take3 Micro-Volume Plates. Absorbance, fluorescence and luminescence measurements are all made using a unique dual-optics design that provides superior performance. The absorbance detection optics uses a xenon flash lamp and monochromator for filter-free wavelength selection from 200 to 999 nm in 1 nm increments. Fluorescence determinations are made using a tungsten halogen lamp with interference filters in conjunction with a PMT detector for maximum sensitivity. Gen5 Data Analysis Software is used for data collection, analysis, exporting and reporting. DETAIL
BioanalyzerThe Agilent Bioanalyzer chip-based capillary electrophoresis machine allows rapid and sensitive investigation of nucleic acid samples including RNA, DNA and proteins. This equipment can be used to provide quality control measurement before downstream experiments such as microarray.DETAIL
Real Time PCR (qPCR) SystemThe system can discriminate up to five targets in a single reaction well with sample volume as low as 10µl. It is a fluorescence-based detection of amplification products through the use of a DNA-binding dye (SYBR Green I) or hybridization probe (TaqMan, for eg. Cy5, FAM, HEX, Texas Red). The initial level of nucleic acid in a reaction can be determined from the number of cycles required to generate a set of amplification products. DETAIL
SpectrophotometerThe DeNovix DS-11 (microvolume) takes the world of microvolume spectrophotometry to a whole new level. Its compact, stand-alone design allows it to operate without a PC and does not require any software installation. Just turn it on and it’s ready to go. DETAIL
High Performance Liquid Chromatography-WatersHigh performance liquid chromatography (HPLC) use a liquid mobile phase to separate the components of a mixture. The components are dissolved in a solvent and forced to flow through a chromatographic column under high pressure. Our HPLC models are designed with two primary configurations which are 2690 and 2690 XE. DETAIL
High Performance Liquid Chromatography-AgilentBrand/Model : Agilent Technologies/1260 Infinity
High Performance Liquid Chromatography (HPLC) has the ability to separate, identify and quantify the compounds that are present in any sample that can be dissolved in liquid.
Centrifugal Partition ChromatographyCentrifugal Partition Chromatography (CPC) is an automated device consists of 250 ml rotor column, pump, injector, detector, fraction collector and Preparative HPLC column channel. Both stationary and mobile phase are liquid. It’s used for purification and isolates the maximum amount of targeted compounds with highest purity in a short time.DETAIL
Flash ChromatographyFlash Chromatography is fully automated device for isolation and purification of samples. It consists of binary pump, injector, detector and fraction collector. The amount of sample load and the ratio of the mobile phase are automatically set according to the Rf value on the TLC analysis of the targeted compounds and the size of column. DETAIL
High Performance Thin Layer ChromatographyHigh Performance Thin Layer Chromatography (HPTLC) is an automated device for developing Thin Layer Chromatography consists of Automatic TLC Sampler, Automatic Developing Chamber, TLC Visualizer, TLC Scanner and TLC-MS Interface. This instrument used for sample purification, sample determination, qualitative and quantitative analysis.DETAIL
Differential Scanning CalorimetryDSC is a thermal analytical instrument used to assess the rate and degree of heat change as a function of temperature and time. The data from this analytical technique will be used to aid in the understanding of the thermal properties of organic and inorganic materials or any of their mixtures. DETAIL
Flame Atomic Absorption SpectrometerFlame Atomic Absorption Spectrometer (FAAS) has the following advanced features:
Cutting-edge fiber optic technology that enhances light throughput.
Fully automated flow-injection (FIAS) system simplifies and speeds up analyses requiring complex sample preparation such as mercury and hydride-forming elements.
Fourier Transform-Infra Red SpectrometerFourier Transform-Infra Red (FTIR) is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. From the spectrum, the functional groups of the sample, compound and material can be determined.DETAIL
Gas Chromatography Mass SpectrometryGas Chromatography Mass Spectrometry (GC/MS) is a combination of 2 instruments; comprising a gas chromatograph (GC) coupled to a mass spectrometer (MS), by which complex mixtures of chemicals may be separated, identified and quantified. In order for a compound to be analysed by GC/MS it must be sufficiently volatile and thermally stable.DETAIL
Liquid Chromatography Mass Spectrometry QTOFInfinityLiquid Chromatography
Mass Spectrometry Quadrupole Time of Flight (LCMS QTOF) is used to analyse and validate targeted compounds, drugs, contaminants and adulterants in herbal preparations, agriculture samples and processed food. The equipment can also be used for analysis of clinical pharmacology and bioanalytical assay in plasma samples.
Melting Point DeterminatorThe melting point determinator (SMP40, Stuart) is a fully automated device built in digital image processing to determine melting point up to three samples simultaneously. The melting point uses digital video imaging technology to detect and capture the melting process of samples.DETAIL
PolarimeterThe Polarimeter P3000 (Kruss) is ease of use and the fastest polarimeter worldwide. The fully automatic measurement caused by passing polarized light through a material that is optically active. It’s used for monitoring of chemical process, purity, quality control; determination of sugar, concentrations, configuration changes; and qualitative and quantitative analysis. DETAIL
Preparative HPLCBrand/Model : Waters
The preparative HPLC is a device that is used to separate, purify and collect organic compounds from complex mixtures. Preparative HPLC will allow collection of sample by a fraction collector for further purification and analysis. The parameters previously employed in analytical chromatography may need to be re-optimized, so as to scale up the process for preparative chromatography.
UV-Vis SpectrophotometerUltraviolet-Visible (UV-Vis) Spectrophotometer uses light in the visible and adjacent (near-UV and near-infrared [NIR]) ranges to measure the wavelength (nm). The wavelength of the ultraviolet(UV) region ranges from 200 to 400 nm, and the visible portion is from 400 to 800 nm.DETAIL
ZetasizerZetasizer Nano ZS is used for the measurement of size, electrophoretic mobility of proteins, zeta potential of colloids and nanoparticles, and optionally the measurement of protein mobility and microrheology of protein and polymer solutions. DETAIL
PROTEAN i12 IEF cell (Bio Rad)The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can fit for 1–12 IPG strips in a single run; using 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the IEF tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.DETAIL
Ettan IPGphor II (Amersham Biosciences)The Ettan™ IPGphor™ II performs first dimension isoelectric focusing (IEF) in 2-dimensional (2-D) electrophoresis. The system comprises of:
– Immobiline™ DryStrip gel strips, which contains an immobilized pH gradient (IPG);
– Strip holders, which serve as holder as well as and IEF chambers for the IPG strips;
– Ettan IPGphor II unit, which includes a high-voltage DC power supply and solid state temperature controller, and can be programmed up to ten user-defined IEF protocols.
The IPGphor II allows for the simultaneous use of up to 12 strips in a single run, subjected to a single program. The maximum length of each strip is at 24 cm.
Mini Protean Tetra cell (Bio Rad)The Mini-PROTEAN Tetra cell is the next-generation mini cell for 1-D vertical gel electrophoresis to separate proteins according to their molecular weight.. This electrophoresis cell can run up to four precast or handcast polyacrylamide gels.DETAIL
SE 600 Ruby (Amersham Biosciences)The SE 600 Ruby system can run up to four gels in a single run. Divider plates convert single-gel sandwich into two-gel club sandwiches.DETAIL
PROTEAN Plus Dodeca cell The PROTEAN Plus Dodeca cell accommodates up to 12 large slab gels, matching the capacity of 1-D runs in the PROTEAN IEF system for high-throughput 2-D applications. The specific features of PROTEAN Plus Dodeca cell such as cooling and recirculating, and its plate electrodes allow consistent and high-resolution results.DETAIL
GS800 Densitometer (Bio Rad)The GS800 Calibrated Imaging Densitometer is a high performance calibrated imaging that converts transparent and opaque electrophoretic samples into digital data. These data are analysed with Quantity One Software operating in Microsoft Window computer system.DETAIL
Pharos FX Plus Laser Scanner (Bio Rad)Molecular Imager System ( Pharos FX Plus Laser Scanner) is a powerful system for complex fluorescence imaging applications. It can be used for the sensitive detection and analysis of DNA, RNA or protein samples in the form of gels, blots or microplates.DETAIL
EXQuest Spot Cutter (Bio Rad)The EXQuest™ spot cutter is a precision instrument that accurately locates and excises protein bands/spots from 1-D and 2-D gels/blots into 96- and 384-well microplates or 96-tube racks for downstream processing and analysis. The EXQuest spot cutter is a fully integrated system controlled through PDQuest™ 2-D analysis software or Quantity One® 1-D analysis software. It consists of an enclosure, an imaging system, a fluidics system, robotics, sensors, a cutting head, a gel tray, a microplate rack, and a wash station.DETAIL
Trans Blot Turbo (Bio Rad) Transfer SystemThe Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system that allows for rapid transfers of protein (as fast as 3 minutes) at high efficiency, as compared to traditional tank protein blotting.DETAIL
Semi Dry Transfer Cell (Bio Rad) The Trans-Blot® SD semi-dry transfer cell allows fast (10-35 minutes), efficient, economical blotting that transfer DNA and RNA using the unique agarose gel semi-dry blotting support frame, without a buffer tank or gel cassettes . It application includes western blotting, nucleic acid transfer northern and northern blot transfer DETAIL
Trans Blot Cell / Trans Blot Plus Cell (Bio Rad)The Trans-Blot® transfer cell is an ideal apparatus for a wide variety of blotting applications. It can be used to run samples in flexible parameters, with power settings ranging from 30 V (overnight transfers) to 200 V in 1 hour (for rapid experiment). The application includes western blotting and nucleic acid transfer.DETAIL
Ultraflextreme MALDI TOF/TOF (Bruker)Ultraflextreme MALDI TOF/TOF in ABI is the latest version of MALDI. These powerful machines have various functions such as protein identification, molecular weight determination, intact protein sequencing and etc. Combination of true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with a 10 bit digitizer will enhance dynamic range and extreme flexibility for a broad variety of complementary research, biopharmaceutical and applied proteomics applications.DETAIL
2100 Bioanalyzer (Agilent)The 2100 Bioanalyzer system is an established automated electrophoresis tool for sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in many different formats for ease-of-use.DETAIL
Electroporator (BioRad)The Gene Pulser Xcell™ system is a modular electroporation system for transfecting any types of cells. Gene Pulser is used to perform electroporation in a microbiology technique through application of electrical field on cells in order to increase the permeability of the cell membrane, that allows chemicals, drugs, or DNA to be introduced into the cell.DETAIL
Real time PCR (BioRad)The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system for gene expression analysis. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive and reliable detection for singleplex or multiplex reactions.DETAIL
Histology Processing System (Leica) Histology is a study on the microscopic structures of cells and tissues of plants and animals. It is often carried out by examining a thin slice (called a ‘section’) of tissue under a light microscope or an electron microscope. 3 main steps involved in tissue processing are called as ‘dehydration’, ‘clearing’ and ‘infiltration’. Each step of the processing method involves the diffusion of a solution into tissue and dispersion of the previous solution in the series. Histology Processing System in ABI is completed with a tissue processor, tissue embedding station, microtome, histology water bath and a bio-imaging navigator microscope. Thus, the histology processing system that available in ABI has the ability to perform the whole work flow of histology study.DETAIL
Automated Karyotyping Automated Karyotyping with a Metasystems IKAROS software is a tool for pairing and ordering processes of all the chromosomes of an organism, thus providing a genome-wide snapshot of an individual’s chromosomes. The system is utilized for Fluorescent in situ hybridization (FISH), both for interphases nuclei and metaphase spreads.DETAIL
Confocal Microscope (Nikon A1+)Confocal microscopy is a versatile imaging method that helps to view cells and tissues in 3D images at an impressive resolution of up to 1.4 times greater than that of conventional microscopy. Confocal microscope has confocal pinholes to allow only light from the plane of focus to reach the detector. It can be used for various applications such as stem cell research, fluorescence resonance energy transfer (FRET, fluorescence recovery after photo-bleaching, fluorescence in-situ hybridization, lifetime imaging, total internal reflection fluorescence microscopy (TIRFM), DNA hybridization, membrane and ion probes, bioluminescent proteins and epitope tagging.DETAIL
Inverted Fluorescence Microscope (Olympus)The inverted microscope system is designed to suit research applications by providing solutions-based software , centering free phase contrast with inversion contrast (IVC) technique. DETAIL
Flow Cytometer (Becton Dickinson)Flow Cytometry is a technique that enables physical and chemical characteristics of cells/ particles to be measured, as they flow past a laser light source in a liquid stream. It measures cellular characteristics such as size and complexity, as well as other parameters, using fluorescent probes. These capabilities have made flow cytometry a powerful tool with multiple applications for researchers and clinicians including analyses for apoptosis (Annexin-V-PI), DNA content, GFP detection and Immunophenotyping.

The Flow Cytometer unit in ABI also can be used for cell sorting works as it is equipped with a BD Influx Fluorescent Activated Cell Sorter (FACS) system. The cell sort feature of BD Influx FACS system allows the physical separation of a specific cell population at high speeds from a heterogeneous group of cells into separate collection test tubes. The unit is equipped with 3 lasers (Blue, Red, UV) and can be adapted to user-defined collection devices. This equipment supports a variety of applications including quick enrichment of rare-event populations.
Aquaculture HatcheryThis aquaculture hatchery is used for artificial breeding, hatching, and rearing through the early life stages of aquatic animals. It keeps larval, brood stock, fresh water shrimp and fish primarily to support the aquaculture industry to reach harvesting size.DETAIL
Cryopreservation Facilities The facilities, both freezer ( -800C) and Cryo tank ( -1960C in liquid Nitrogen), are set at very low temperature to store and preserve any samples at long period of time. DETAIL
In Vitro Fertilization (IVF) Facilities In vitro fertilization (IVF) involves fertilization outside the body in an artificial environment. It is a type of assisted reproductive technology (ART) that involves surgical procedures to help sperm fertilize an egg. These ABI facilities cover Intracytoplasmic Sperm Injection (ICSI) facilities, inverted fluorescent microscope with micromanipulators, stereomicroscope, biological safety cabinet, CO2 Incubator and cryopreservation facilities.DETAIL
Biosafety Level 2 (Advance) Laboratory This facility provide clean working environment that mainly used to minimize contamination risk for cell culture or microbial research that requires high aseptic technique. This room is completed with media preparation room, biological safety cabinet, anaerobic chamber, laminar flow, -200C freezer, autoclave and microscope.DETAIL
PulverizerPulverizer is used to break feed raw materials into fine powderDETAIL
MixerMixer is used to thoroughly mix the required quantity of feed raw materials.DETAIL
ExtruderExtruder is used to extrude the mixed feed raw materials into feed pellet form.DETAIL
Drying oven Drying oven is used to dry the feed pelletDETAIL
Coating machineCoating machine is used to coat heat-sensitive feed materials and oil on the surface of the feed pellet.DETAIL
Gas Chromatography / Mass Spectrometry Triple Quadrupole (GC MS/MS-QQQ) (BRUKER)GC/MS combines the features of gas-chromatography (separation) and mass spectrometry (detection) to identify volatile compounds, as well as for quantitative analysis within a test sample. Applications of GC/MS include fatty acid analysis, alcohol and hydrocarbon analyses, and also other volatile samples. Samples with poor volatility and thermal stabilities require derivatization to make them suitable for GC/MS analysis. Recent developments in GC/MS hardware have resulted in excellent sensitivity for targeted compounds with lower detection limits.DETAIL
Gas Chromatography- Flame Ionization Detector (FID) (AGILENT)GC-FID uses flame ionization detector (FID) to measure the concentration of organic species in a gas stream. The operation of FID is based on the detection of ions that is formed during combustion of organic compounds in hydrogen flame. Applications of GC-FID include fatty acid analysis, alcohol and hydrocarbon analyses, and also other volatile samples. Samples with poor volatility and thermal stabilities require derivatization to make them suitable for GC/MS analysis. DETAIL
High Performance Liquid Chromatography (HPLC) (DIONEX)HPLC is used to separate, identify, and quantify non-volatile components in samples for research purposes. pharmaceutical drug development and any other related analytical works. Solvent containing the components pass through a stationary column and leading to a separation of the components as they flow out of the column. This HPLC equipped with a fraction collector for sample collection through extract fractionation based on the compound peak and mass with the use of Chromeleon software. Isolation and purification of compound can be performed from micro-gram to lower-milligram quantities. DETAIL
6550 iFunnel Liquid Chromatography/Mass Spectrometry – Quadrupole Time of Flight (LC/MS-QToF) (Agilent)Liquid chromatography–mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (The 1290 Infinity II LC) with the mass analysis capabilities of mass spectrometry (iFunnel MS-QToF). This technique is performed on liquid form of samples with the presence of metabolites that thermally unstable, large, polar, ionic or non-volatile. The Agilent 1290 Infinity II LC system delivers ultra-fast separation and detection performance that maximize analytical efficiency at the highest quality. The high sensitivity of the 6550 iFunnel Q-TOF LC/MS allows for highly repetitive qualitative analyses by providing accurate molecular weight up to five decimal points, and repetitive quantitative analyses at trace level up to femtogram-level sensitivity. This MS-QToF also has the capability for structure elucidation through MS/MS fragmentation of compounds. The LC/MS-QTOF is equipped with METLIN Database for determination of metabolites ID.DETAIL
Ultra Performance Liquid Chromatography (UPLC) (Waters)UPLC works in a similar principle to HPLC that involves separation, identification, and quantification of non-volatile components in samples. UPLC provides faster separation (50x faster) than conventional HPLC, due to shorter column and lower flow rate of UPLC. Applications of UPLC include separation, identification and quantification of active compound in samples for research purposes. pharmaceutical drug development and any other related analytical works.DETAIL
UV-VIS Spectrophotometer (PERKIN ELMER)Ultraviolet–visible (UV-Vis) spectrophotometer refers to an absorption spectrophotometer in the ultraviolet-visible spectral region. Absorbance of a solution is directly proportional to the concentration of the absorbing species in the solution and the path length UV-Vis. UV-Vis spectrophotometer is routinely used in analytical chemistry for the quantitative determination of different analytes. UV-Vis can be applied to determine the kinetics or rate constant of a chemical reaction. It is equipped with gridless PMT detector, LAMBDA 850 which provides excellent sensitivity between 175 and 900 nm, with resolution up to 0.05 nm.DETAIL
Nuclear Magnetic Resonance (NMR) (AGILENT)Nuclear Magnetic Resonance (NMR) spectroscopy is an analytical chemistry technique used for determining the content and purity of a sample as well as its molecular structure. NMR spectroscopy also provides identification, reaction state, and chemical environment of molecules. NMR spectroscopy is the definitive method to identify unknown monomolecular, organic compounds, proteins and other complex molecules. DETAIL
Fourier Transform Infra-Red (FTIR) (PERKIN ELMER)Fourier transform infrared (FTIR) is the preferred method of infrared spectroscopy. When IR radiation is passed through a sample, some radiation is absorbed by the sample and some passes through (is transmitted). The resulting signal at the detector is a spectrum representing a molecular ‘fingerprint’ that can be used to screen and scan samples for many different components. FTIR is an effective analytical instrument for detecting functional groups for component identification in samples.DETAIL
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) (AGILENT)Inductively coupled plasma – mass spectrometry (ICP-MS) is a type of mass spectrometry that uses an Inductively coupled plasma to ionize the sample. It atomizes the sample and creates atomic and small polyatomic ions, which are then detected. It has the ability to detect metals and several non-metals in liquid samples at very low concentrations. It can detect different isotopes of the same element, which makes it a versatile tool in Isotopic labeling. Compared to atomic absorption spectroscopy, ICP-MS has greater speed, precision, and sensitivity. One of the largest applications of ICP-MS is in the food safety, medical and forensic specifically, toxicology.DETAIL
High Pressure Processing (Avure)High Pressure Processing (HPP) is a cold pasteurization technique by which products, already sealed in its final package, are introduced into a vessel and subjected to a high pressure (300–600MPa) transmitted by water. The high pressure is able to inactivate the vegetative flora (bacteria, virus, yeasts, moulds and parasites) in food, extending the shelf life and guaranteed safety of products. HPP respects the sensorial and nutritional properties of food, because of the absence of heat treatment, and maintains its original freshness throughout the shelf-life.DETAIL
Integrated Centrifugal Partition Chromatography- Spot Prep II HPLC System (Armen Instrument)Centrifugal Partition Chromatography (CPC) is a purification technique that based on the principles of partitioning chromatography, with the use of two immiscible liquid phases. Centrifugal partition chromatography does not use any solid stationary phase, therefore guarantees for good separation and high productivity. CPC is the ideal technique for isolation of active compounds from crude natural product extracts since it enables fast separations, eliminates the use of silica, reduces the amount of solvent used, enables 100% recovery of the analytes, and can be easily scaled up. Major pharmaceutical and cosmetics companies worldwide use this device in their research and development work. CPC allows injection up to 100g of sample extract and achieve purification more than 99% and recovery above 95%. The system is equipped with 50 mL/min, 300 bar quaternary gradient pump, UV-Vis DAD detector and Prep software for development of HPLC system.DETAIL
Microplate Reader (Thermo Scientific)Microplate reader is an instrument used for biological, chemical or physical events of samples in microtiter plates. It can be used to detect and quantify samples/cells concentration in several biological and chemical assays. They are widely used in research, cytotoxicity study, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. DETAIL
Pulsed Electric Field (Diversified Technologies Inc.)Pulsed electric fields (PEF) is a non-thermal method of food preservation that uses short pulses of electricity for microbial inactivation with only minimal detrimental effect on food quality attributes. PEF technology involves the application of high voltage pulses to liquid or semi-solid food between two electrodes. Electric fields in the range of 5-50 kV/cm generated by short high voltage pulses (µs) between two electrodes can cause microbial inactivation at a temperature below the thermal processing application. DETAIL
Kjeltec by FOSSThe Kjeldahl method or Kjeldahl digestion is a method for quantitative determination of nitrogen contained in organic substances. The Kjeldahl method is an internationally recognized method for estimating protein content in foods. It is also used in the analyses of soil, waste water, fertilizer and other materials. Kjeltec by Foss automates and speeds the digestion and distillation process in Kjeldahl method for fast result. DETAIL
Soxtherm Rapid Soxhlet System by GerhardtSoxhlet extraction is used for lipid extraction from a solid material. The Gerhardt Soxtherm Rapid Soxhlet System can automate and speed the soxhlet extraction process, and able to evaporate as well as recover the solvent. By immersing sample in hot solvent, the extractable material is removed more quickly than the traditional Soxhlet extraction method. Soxtherm Rapid Soxhlet System is controlled by Soxtherm Manager software. DETAIL
Fast Protein Liquid Chromatography (AKTA PURIFIER 9000)Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation by FPLC is possible because different components of a mixture have different affinities for two materials, a moving fluid (the “mobile phase”) and a porous solid (the stationary phase). DETAIL
Capillary Electrophoresis (BECKMANN COULTER)Capillary electrophoresis employs narrow-bore (20-200 µm i.d.) capillaries to separates ions from both large and small molecules based on their electrophoretic mobility, facilitated with the use of high voltages. The electrophoretic mobility is dependent upon the charge of the molecule, the viscosity, and the atom’s radius. This system features a modular high sensitivity selectable wavelength UV detector, which allows wavelength changes during a run. It is used most predominantly because it gives faster results and provides high resolution separation. Capillary electrophoresis (CE) is a widely used technique in biosciences, which includes DNA fingerprinting, drug analysis and characterization of proteins.DETAIL
Rheometer(TA INSTRUMENT)Rheometer is a laboratory device used to measure the way in which a liquid, suspension or slurry flows in response to applied forces. Unlike a viscometer, which can only measure the viscosity of a fluid under a limited range of conditions, a rheometer is capable of measuring viscosity and elasticity of non-Newtonian materials under a wide range of conditions. Application in rheometer including food product development with specific rheological properties. DETAIL
Differential Scanning Calorimeter (Perkin Elmer)Differential scanning calorimeter (DSC) is a thermal analysis technique that particularly suitable for food analyses (both in the liquid and solid forms) as food are often subject to heating or cooling conditions during processing. The calorimetric information from DSC can be directly used to understand the thermal transitions that the food system may undergo during processing or storage. DETAIL
Microwave Digester (Milestone)Microwave digestion is a technique used to dissolve heavy metals prior to analysis by inductively coupled plasma, atomic absorption, or atomic emission measurements. This technique is usually accomplished by exposing a sample to a strong acid through microwave irradiation at high pressure and temperature in a closed vessel. Increase in temperature and pressure of the low pH sample medium increases the speed of thermal decomposition of the sample and the solubility of heavy metals in solution. The dissolved heavy metals are quantified through elemental techniques.DETAIL
Spray Dryer (BUCHI)Spray drying is a method of producing dry powder from a liquid or slurry samples by rapidly drying process with the use of hot gas. It separates the solute or suspension as solid powder and discard the solvent in a vapor form. The liquid stream is sprayed through a nozzle into a hot vapor stream and vaporized. Solid powder is formed as moisture quickly leaves the droplets. The solid is collected in a drum or cyclone. Spray drying is often used as an encapsulation technique by food and other industries. This is the preferred drying method for some food and pharmaceuticals products. DETAIL
Freeze dryer 4 L & 6 L (Labconco)Freeze-drying or lyophilization, is a technique that applies very low temperature and low pressure to remove water content from any samples such as food, plants, animals, in raw form or water-based extract form, that allow long preservation of samples. DETAIL
Rotary Evaporator (BUCHI)Rotary evaporator use evaporation approach to remove solvent from sample for efficiently in a gentle way. Liquid solvents can be removed without excessive heating of thermal-sensitive components. Rotary evaporation is most often and conveniently applied to separate active compound in solvent after solvent extraction of any samples.DETAIL